IT-LIST Digest 40Topics covered in this issue include:  1) How to read object edge coordinates	by hubert@bert.van.forintek.ca  2) Re: object tracking?	by Linnea Ista   3) Re: ImageTool stacks and .its format	by "S. Brent Dove"   4) Re: How to read object edge coordinates	by "Dr. Bin Yu"   5) Re: How to read object edge coordinates	by "S. Brent Dove"   6) ImageTool 1.25 + DT3152 + Windows 95 ??????	by Soonsung Hong   7) Re: ImageTool 1.25 + DT3152 + Windows 95 ??????	by "BRYAN J. CROSBY"   8) Re: NEW ImageTool Version 1.25	by Alvaro Castro - Profesor Facultad Odontologia   9) Re: ImageTool 1.25 + DT3152 + Windows 95 ??????	by Linnea Ista  10) ImageTool Developers List Server	by "S. Brent Dove"  11) Analysis of PAGE data from IT	by Andy Phillips  12) Re: How to read object edge coordinates	by "Don Wilcox" ----------------------------------------------------------------------Date: Tue, 27 Aug 1996 09:22:29 +0000From: hubert@bert.van.forintek.caTo: it-list@sparky.uthscsa.eduSubject: How to read object edge coordinatesMessage-ID: <9608271818.AA27828@sparky.uthscsa.edu>Is it possible to read or obtain the x,y coordinates of the perimeter (edges)of the objects detected ?Our goal would be to characterize typical shapes of the objects.Thank you------------------------------Date: Tue, 27 Aug 1996 12:45:40 -0600 (MDT)From: Linnea Ista To: it-list@sparky.uthscsa.eduSubject: Re: object tracking?Message-ID: On Tue, 27 Aug 1996, Shaibani, Sanan wrote:> Is it possible to register, and then track the path of multiple objects in a> sequence of gray scale images to determine the distance the objects has> moved through 15 consecutive frames? > > I am using IT 1.25 and have yet to determine how use the stack function to> link information from one frame to the next.> > Any suggestions or comments will be appreciated greatly.This would be very helpful for me too!Linnea> > ------------------------------Date: Tue, 27 Aug 1996 14:00:02 -0500 (CDT)From: "S. Brent Dove" To: IT List Subject: Re: ImageTool stacks and .its formatMessage-ID: <01I8RU4CSD54007NEH@uthscsa.edu>Recently ITList,>I recently downloaded ImageTool from the FTP site. I want to use it to >display>image sequences which were created by a TI thermal imager. Each image is>240 rows x 320 columns x 2 bytes per pixel, at a 30 Hz frame rate. These >images>are stored row wise in a binary data file. I usually view them with Matlab.>>I read the documentation with ImageTool and found a general description of >the >.its format for a stack. This sounds like what I need. I would like to >somehow >define my image format in the stack and then define the 200 or so images >to be >shown as text path names to the individual images with a text editor.  The >documentation says this can be done, but supplies no example. Does anyone >know how to do this?The format for the text-based stacks format is as follows:First line of text file indicates it is a text based stackSecond line is optional and relates the frame rate of playback Third line is also optional and relateds to the spacing of a 3D data set.Fourth  defines file sectionRemaining lines indicate the files to be loaded and the order. Complete paths are requiredExample_Txtframes per second=12 (optional)spatial separation=2 (optional)filesC:\UTHSCSA\ImageTool\Images\pic1.tifC:\UTHSCSA\ImageTool\Images\pic2.tifC:\UTHSCSA\ImageTool\Images\pic3.tifC:\UTHSCSA\ImageTool\Images\pic4.tifC:\UTHSCSA\ImageTool\Images\pic5.tifC:\UTHSCSA\ImageTool\Images\pic6.tifC:\UTHSCSA\ImageTool\Images\pic7.tifC:\UTHSCSA\ImageTool\Images\pic8.tifC:\UTHSCSA\ImageTool\Images\pic9.tifC:\UTHSCSA\ImageTool\Images\pic10.tifThis format of stacks only supports ImageTool formats (tif, bmp, pcx, etc) this does not support imported files.  Therefore your images must first me converted to 16 bit TIF files.I hope that this helped. So long for now.S. Brent Dove                            Voice: (210) 567-3333Diagnostic Sciences                      Fax:   (210) 567-3334University of Texas                      Email: dove@uthscsa.eduHealth Science Center                    Web:   ddsdx.uthscsa.eduSan Antonio, TX    USA                   ftp:   maxrad6.uthscsa.edu------------------------------Date: Tue, 27 Aug 1996 15:20:28 -0400From: "Dr. Bin Yu" To: it-list@sparky.uthscsa.eduSubject: Re: How to read object edge coordinatesMessage-ID: <32234A7C.7653@cps.msu.edu>hubert@bert.van.forintek.ca wrote:> > Is it possible to read or obtain the x,y coordinates of the perimeter (edges)> of the objects detected ?> > Our goal would be to characterize typical shapes of the objects.> > Thank youYou can save the results (ASCII) of analysis to a file.------------------------------Date: Tue, 27 Aug 1996 17:00:39 -0500 (CDT)From: "S. Brent Dove" To: IT List Subject: Re: How to read object edge coordinatesMessage-ID: <01I8S0FE7FMI006YWL@uthscsa.edu>Recently on ITlist>Is it possible to read or obtain the x,y coordinates of the perimeter (edges)>of the objects detected ?>>Our goal would be to characterize typical shapes of the objects.In the new version 1.25 of ImageTool x, y coordinates of the outline of found object can be output to a text file for analysis by other software.In order to perform this process, first select the blob.tif image to work on. Make sure that Show object outlines on original image is selected on the Find Objects preferences sheet uder the Settings menu.  Next select the Find Objects item form the Analysis menu.  Next select Manual for the thresholding method. Using the sliders select a threshold that clearly defines the objects that you want the outlines of. After thresholding the image the objects should be outlined and numbered in the original image. Next select the Save Object Points from the Analysis menu.  You will be prompted with a standard Save diolog.  Type in the name of a text file that will contain the x,y coordinates of the object outines.The format of the text file is x and y coordinates separated by comma and each coordinate pair separated by a space. Each object outline is separated by a return.You can then load these values into Excel or use another program to characterize typical shapes.S. Brent Dove                            Voice: (210) 567-3333Diagnostic Sciences                      Fax:   (210) 567-3334University of Texas                      Email: dove@uthscsa.eduHealth Science Center                    Web:   ddsdx.uthscsa.eduSan Antonio, TX    USA                   ftp:   maxrad6.uthscsa.edu------------------------------Date: Tue, 27 Aug 1996 21:08:49 -0500From: Soonsung Hong To: it-list@sparky.uthscsa.eduCc: hongsoon@egr.msu.eduSubject: ImageTool 1.25 + DT3152 + Windows 95 ??????Message-ID: <3223AA31.5174@egr.msu.edu>Thanks for your efforts for the ImageTool 1.25.ImageTool 1.25 doesn't recognize my frame grabber (DT3152) under theWindows 95. I couldn't find any alias of grabber (DT3152$0) at Setting/Preference/ DTOL Configure/ Device list. I already checked the setup ofDT3152 and DTOL at Control Panel of Windows 95.What seems to be the problem?Please, let me know.Thanks,---------------------------------------------------------------------Soonsung Hong        hongsoon@egr.msu.eduDept of Material Science and MechanicsMichigan State University---------------------------------------------------------------------------------------------------Date: Wed, 28 Aug 1996 12:35:43 GMTFrom: "BRYAN J. CROSBY" To: it-list@sparky.uthscsa.eduSubject: Re: ImageTool 1.25 + DT3152 + Windows 95 ??????Message-ID: Dear  Soonsung> ImageTool 1.25 doesn't recognize my frame grabber (DT3152) under the> Windows 95. I couldn't find any alias of grabber (DT3152$0) at Setting/> Preference/ DTOL Configure/ Device list. I already checked the setup of> DT3152 and DTOL at Control Panel of Windows 95.> > What seems to be the problem?> Please, let me know.Well It should not be the latest copy of IT 1.25 as I also have a dt3152 on Win 95 and it is always recognized. I have even got images, however at present IT does not program for non-interlaced cameras.(bug fix coming soon I bet)I suggest that you check that the PCI plug and play feature is working correctly. Have you installed the device driver correctly for your operating system(have to buy a different one for either  Win NT or 95) For example can you grab an image using the Acq2host program which comes with the device drivers and SDK for DT3152?Happy hunting, but it sounds like a system problem.sincerley yoursBryan J. CrosbyIRC in Polymer Science and TechnologyUniversity of LeedsLeeds LS2 9JTPh :Lab & Office :+44(0)113 2333 904    Fax          :+44(0)113 2333 846Email phybjc@gps.leeds.ac.uk   or B.J.Crosby@leeds.ac.uk------------------------------Date: Wed, 28 Aug 1996 07:18:20 -0500 (EST)From: Alvaro Castro - Profesor Facultad Odontologia To: it-list@sparky.uthscsa.eduSubject: Re: NEW ImageTool Version 1.25Message-ID: Dear Brent,Congratulations for you and  your team.We are using both IT and  IQ,  At the present time we are  comparing the IT and conventional reading  for measuring  the tomography cuts  for implants.Cordially,Alvaro Castro , DDSProfesor de RadiologiaFacultad de OdontologiaUniversidad JaverianaSantafe de Bogota, DC, Colombia, SAe-mail : acastro@javercol.javerana.edu------------------------------Date: Wed, 28 Aug 1996 09:01:22 -0600 (MDT)From: Linnea Ista To: it-list@sparky.uthscsa.eduSubject: Re: ImageTool 1.25 + DT3152 + Windows 95 ??????Message-ID: On Tue, 27 Aug 1996, Soonsung Hong wrote:> Thanks for your efforts for the ImageTool 1.25.> > ImageTool 1.25 doesn't recognize my frame grabber (DT3152) under the> Windows 95. I couldn't find any alias of grabber (DT3152$0) at Setting/> Preference/ DTOL Configure/ Device list. I already checked the setup of> DT3152 and DTOL at Control Panel of Windows 95.> > What seems to be the problem?> Please, let me know.> > Thanks,> > > ---------------------------------------------------------------------> Soonsung Hong        hongsoon@egr.msu.edu> > Dept of Material Science and Mechanics> Michigan State University> ---------------------------------------------------------------------> Hi!I had the same problem with my DT3155. It turns out that, despite assurances to the contrary when I ordered the frame grabber card, you need the SDK program to aquire images using IT. I hope this helps!Linnea ------------------------------Date: Wed, 28 Aug 1996 10:05:11 -0500 (CDT)From: "S. Brent Dove" To: IT Announce ,Subject: ImageTool Developers List ServerMessage-ID: <01I8T07EFBMU00029Y@uthscsa.edu>Hello ImageTool Users,We have determined that the IT-ANNOUNCE list server really does not have any function since most users subscribe to the IT-LIST.  So this list server function has been discontinued.  All information on new releases will only be made on the IT-LIST.In order to better meet the needs of ImageTool developers we have initiated a new list server specifically designed to meed these needs.  Information in this forum will be primary directed at the development of plug-in using ImageTool.Please use the appropriate list server for the information you need regarding the use or development of ImageTool.  To subscribe to the new ImageTool Developers List Server:        - Address an email message to:  listproc@sparky.uthscsa.edu        - Leave the Subject line blank.        - In the body of the message, enter the following command:                subscribe  IT-DEV  yourfirstname yourlastname         S. Brent Dove                            Voice: (210) 567-3333Diagnostic Sciences                      Fax:   (210) 567-3334University of Texas                      Email: dove@uthscsa.eduHealth Science Center                    Web:   ddsdx.uthscsa.eduSan Antonio, TX    USA                   ftp:   maxrad6.uthscsa.edu------------------------------Date: Wed, 28 Aug 1996 17:14:53 +0100From: Andy Phillips To: it-list@sparky.uthscsa.eduCc: andy.phillips@bbsrc.ac.ukSubject: Analysis of PAGE data from ITMessage-ID: <3224707D.5454@bbsrc.ac.uk>Hi,I'm looking for software to help analyze my autorads of Northern blots. I downloaded ImageTool and found the function for producing a line profile and for saving the data to file. Now I need a program that can read the data from the line profiles and integrate the area under each peak, with the facility to set the background level. Can anyone suggest software to achieve this?Thanks.Andy Phillips ------------------------------------------------------------------------  Email  : andy.phillips@bbsrc.ac.uk  : University of Bristol Home   : andy@cycad.demon.co.uk     : IACR Long Ashton Research Station  Phone  : +44-1275-549257            : Long Ashton Fax    : +44-1275-394281            : Bristol, BS18 9AF, UK WWW    : http://www.lars.bbsrc.ac.uk/plantsci/molbiol/andy.html ------------------------------------------------------------------------------------------------------Date: Wed, 28 Aug 1996 14:01:01 -0700From: "Don Wilcox" To: Subject: Re: How to read object edge coordinatesMessage-ID: <199608282114.OAA14730@dogbert.xroads.com>----------> From: hubert@bert.van.forintek.ca> To: wilcox@xroads.com> Subject: How to read object edge coordinates> Date: Tuesday, August 27, 1996 12:23 AM> > Is it possible to read or obtain the x,y coordinates of the perimeter(edges)> of the objects detected ?> > Our goal would be to characterize typical shapes of the objects.It is possible, using version 1.20 or later, to extract the objectinformation in a plug-in, so you can write analysis plug-ins that make useof the object set.Don------------------------------End of IT-LIST Digest 40